Abstract
We have isolated cDNAs from normal human breast tissue and breast tumour cells that encode a protein (pez) with features of a novel non-receptor tyrosine phosphatase possessing N-terminal sequence homology to the ezrin-band 4.1-merlin-radixin protein family. Northern blot analysis indicates that pez is expressed in a variety of human tissues including kidney, skeletal muscle, lung and placenta. Fluorescence in situ hybridization has mapped pez to chromosome 1 region q32.2-41. Sequence identity to a characterized polymorphic marker confirms this localization.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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Breast / metabolism*
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Breast Neoplasms / metabolism*
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Chromosome Mapping
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Chromosomes, Human, Pair 13*
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Cloning, Molecular
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Cytoskeletal Proteins
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DNA Primers
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DNA, Complementary / metabolism*
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Female
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Gene Library
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Humans
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In Situ Hybridization, Fluorescence
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Molecular Sequence Data
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Phosphoproteins / biosynthesis*
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Phosphoproteins / chemistry
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Polymerase Chain Reaction
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Protein Tyrosine Phosphatases / biosynthesis*
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Protein Tyrosine Phosphatases / chemistry
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Protein Tyrosine Phosphatases / physiology
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Protein Tyrosine Phosphatases, Non-Receptor
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RNA, Messenger / isolation & purification
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RNA, Messenger / metabolism
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Sequence Homology, Amino Acid
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Tumor Cells, Cultured
Substances
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Cytoskeletal Proteins
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DNA Primers
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DNA, Complementary
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Phosphoproteins
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RNA, Messenger
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ezrin
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PTPN14 protein, human
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Protein Tyrosine Phosphatases
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Protein Tyrosine Phosphatases, Non-Receptor