Abstract
A HEp-2 cell-vacuolation factor was extracted and purified from the culture supernatant of a Bacillus cereus strain which caused emetic-syndrome food poisoning. The final preparation was chemically pure, and the toxin was named as cereulide. Mass spectrometry, NMR studies and chemical degradation revealed that the cereulide is a cyclic dodecadepsipeptide, (D-O-Leu-D-Ala-L-O-Val-L-Val)3, which is closely related to the potassium ionophore, valinomycin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacillus cereus / chemistry*
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Bacillus cereus / pathogenicity
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Bacterial Toxins / chemistry
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Bacterial Toxins / toxicity*
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Cells, Cultured / pathology
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Depsipeptides*
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Foodborne Diseases / etiology
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Humans
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Magnetic Resonance Spectroscopy
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Mass Spectrometry
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Molecular Sequence Data
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Peptides, Cyclic / chemistry
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Peptides, Cyclic / toxicity*
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Vacuoles
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Virulence
Substances
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Bacterial Toxins
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Depsipeptides
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Peptides, Cyclic
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cereulide