Abstract
Gicerin is an integral membrane glycoprotein of about 82 kd that is transiently expressed in the developing CNS. Gicerin was first identified as a binding protein for neurite outgrowth factor (NOF), a member of the laminin family of extracellular matrix proteins. By isolating and sequencing a gicerin cDNA, we have found that this protein is a novel member of the immunoglobulin superfamily. The deduced protein (584 amino acids) consists of five immunoglobulin-like loop structures in an extracellular domain, a single transmembrane region, and a short cytoplasmic tail. Cells transfected stably with gicerin cDNA adhered to NOF and aggregated with each other, indicating that gicerin exhibits both heterophilic and homophilic adhesion activities.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Avian Proteins*
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Base Sequence
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Blotting, Northern
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CD146 Antigen
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Carrier Proteins / chemistry
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism
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Cell Adhesion Molecules / chemistry
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Cell Adhesion Molecules / genetics*
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Cell Adhesion Molecules / metabolism
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Cell Line
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Chick Embryo
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Chickens
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Cloning, Molecular*
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Consensus Sequence
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DNA, Complementary / chemistry
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DNA, Complementary / isolation & purification
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Fibroblasts / metabolism
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Gene Expression*
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Immunoblotting
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Immunohistochemistry
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Mice
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Molecular Sequence Data
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Nerve Growth Factors / metabolism*
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Transfection
Substances
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Avian Proteins
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CD146 Antigen
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Carrier Proteins
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Cell Adhesion Molecules
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DNA, Complementary
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Mcam protein, mouse
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Nerve Growth Factors
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gicerin protein, Gallus gallus