Guinea-pig neutrophil cationic peptides are encoded by at least three kinds of mRNA transcripts

Comp Biochem Physiol B. 1993 Oct;106(2):387-90. doi: 10.1016/0305-0491(93)90317-x.

Abstract

1. cDNA clones encoding antimicrobial guinea-pig neutrophil cationic peptides, GNCP-1 and GNCP-2 were isolated from a bone marrow cell cDNA library. 2. Three kinds of cDNAs (GNCP-1A, GNCP-1B and GNCP-2 cDNAs) were obtained, and the analysis of these cDNAs indicated that GNCPs were produced as precursor proteins comprising 93 amino acid residues, which were composed of signal sequences (N-terminal 19 residues), pro-peptide sequences (43 residues) and mature GNCP sequences (31 residues). 3. The deduced amino acid sequences showed that GNCP-1A and GNCP-1B differed by only one amino acid substitution in the pro-peptide region, and GNCP-1B and GNCP-2 differed by only one amino acid substitution in the mature peptide region. The nucleotide sequences of these cDNAs were highly homologous (99%). 4. Together these observations indicate that GNCPs are encoded by at least three kinds of mRNA transcripts which are likely derived from the similar genes.

Publication types

  • Comparative Study

MeSH terms

  • Alleles
  • Animals
  • Cloning, Molecular
  • DNA, Complementary / genetics*
  • Gene Library
  • Genetic Code*
  • Guinea Pigs / blood*
  • Molecular Sequence Data
  • Neutrophils / physiology*
  • RNA, Messenger / genetics*
  • Sequence Homology, Nucleic Acid

Substances

  • DNA, Complementary
  • RNA, Messenger

Associated data

  • GENBANK/D14118