Preliminary crystallographic studies of crotin II

M H Chen; K J Zhou; Z J Fu; K Z Pan

doi:10.1006/jmbi.1993.1641

Preliminary crystallographic studies of crotin II

J Mol Biol. 1993 Dec 5;234(3):908-9. doi: 10.1006/jmbi.1993.1641.

Authors

M H Chen¹, K J Zhou, Z J Fu, K Z Pan

Affiliation

¹ Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou.

PMID: 8254687
DOI: 10.1006/jmbi.1993.1641

Abstract

Crotin II isolated from the seeds of Euphorbiacea Croton tiglium has been crystallized. Crystals were grown by vapor diffusion using KCl as the precipitant. The crystal of crotin II belongs to the space group P6(1) or P6(5) with cell parameters a = b = 94.62 A, c = 28.43 A, alpha = beta = 90 degrees, gamma = 120 degrees. The asymmetric unit contains one molecule of 15,000 Da. A data set to 1.82 A has been collected on an area detector.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Crystallization
Crystallography, X-Ray
Plant Proteins / chemistry*
Plant Proteins / isolation & purification
Protein Conformation
Protein Synthesis Inhibitors / chemistry*
Protein Synthesis Inhibitors / isolation & purification
Ribosome Inactivating Proteins, Type 1
Ribosomes / drug effects
Seeds

Substances

Plant Proteins
Protein Synthesis Inhibitors
Ribosome Inactivating Proteins, Type 1
crotin II protein, Croton tiglium