Abstract
CTP:phosphocholine cytidylyltransferase is the rate-controlling enzyme in phosphatidylcholine biosynthesis and is essential for the survival of eukaryotic cells. The murine cDNA for the cytidylyltransferase was cloned and sequenced. A genomic clone was isolated and the chromosomal location of the Ctpct locus determined by Southern blot hybridization of DNAs from a panel of interspecific backcross progeny derived from matings of [(C57BL/6J x Mus spretus)F1 x C57BL/6J] mice. These data place the Ctpct gene on mouse chromosome 16 between the Smst and Stf-1 genes.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Choline-Phosphate Cytidylyltransferase
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Chromosome Mapping*
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Crosses, Genetic
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Female
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Genomic Library
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Male
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Mice
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Mice, Inbred C57BL / genetics*
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Molecular Sequence Data
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Muridae / genetics*
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Nucleotidyltransferases / genetics*
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Polymerase Chain Reaction
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Restriction Mapping
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics
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Sequence Homology, Amino Acid
Substances
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Nucleotidyltransferases
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Choline-Phosphate Cytidylyltransferase