Abstract
Expression of the gene for cysteine-rich secretory protein-1 (CRISP-1), the counterpart of the rat sperm-coating protein DE/AEG, was evidenced in male mouse salivary gland by both isolation of the corresponding cDNA clone and RNA blot analysis. In addition, the cDNA coding for the new related CRISP-3, which shares 77% amino acid identity with CRISP-1, was isolated from a salivary gland cDNA library. The activity of CRISP-1 and -3 genes in mouse salivary gland is strongly androgen dependent, as seen in castrated males; also, no CRISP-1 and only weak CRISP-3 expression was detected in female salivary gland. A variety of tissues were tested for the presence of CRISP-3 transcripts, but specific signals were only found in salivary gland. The results of genomic DNA blot analysis are compatible with the existence of single discrete genes for CRISP-1 and CRISP-3.
MeSH terms
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Amino Acid Sequence
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Androgens / pharmacology*
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Animals
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Base Sequence
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Blotting, Northern
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Blotting, Southern
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Cloning, Molecular
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DNA / chemistry
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DNA / genetics
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Female
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Gene Expression / drug effects*
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Male
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Membrane Glycoproteins*
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Mice
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Molecular Sequence Data
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RNA, Messenger / analysis
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Salivary Glands / metabolism*
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Salivary Proteins and Peptides / chemistry
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Salivary Proteins and Peptides / genetics*
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Seminal Plasma Proteins*
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Sequence Homology
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Tissue Distribution
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Transcription, Genetic*
Substances
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Androgens
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CRISP3 protein, human
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Crisp1 protein, mouse
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Crisp3 protein, mouse
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Crisp3 protein, rat
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Membrane Glycoproteins
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RNA, Messenger
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Salivary Proteins and Peptides
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Seminal Plasma Proteins
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DNA
Associated data
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GENBANK/L05559
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GENBANK/L05560
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GENBANK/L09679
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GENBANK/L09680
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GENBANK/L09681
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GENBANK/L09682
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GENBANK/L09683
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GENBANK/L16554
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GENBANK/L16555
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GENBANK/L16556