Cloning and expression of a rat cytochrome P-450 11 beta-hydroxylase/aldosterone synthase (CYP11B2) cDNA variant

Biochem Biophys Res Commun. 1993 Jul 15;194(1):112-7. doi: 10.1006/bbrc.1993.1792.

Abstract

A variant cytochrome P450 11 beta-hydroxylase/aldosterone synthase cDNA (CYP11B2) clone, pALDO-4, was isolated and sequenced from a rat zona glomerulosa cDNA library. This clone contains a 1521 bp open reading frame coding for a 506 amino acid residue protein which has a 30 amino acid extension peptide which is 6 residues longer than that reported by others. Seven nucleotide mutations in the coding region of the mature protein were found in comparison to the reported cDNAs. These mutations resulted in three amino acid changes in the mature protein which were identical to those present in the cytochrome P-450 11 beta-hydroxylase (CYP11B1). Two nucleotide substitutions and two nucleotide insertions were also identified in the 3'-noncoding region. The expression product of this cDNA transfected into COS7 cells converted DOC to aldosterone, corticosterone and 18-hydroxycorticosterone less efficiently than the plasmid containing the cDNA with normal 24 amino acid extension peptide.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular / methods
  • Cytochrome P-450 CYP11B2
  • Cytochrome P-450 Enzyme System / biosynthesis
  • Cytochrome P-450 Enzyme System / genetics*
  • Cytochrome P-450 Enzyme System / metabolism
  • Kinetics
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Polymerase Chain Reaction / methods
  • RNA, Messenger / isolation & purification
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Steroid Hydroxylases / biosynthesis
  • Steroid Hydroxylases / genetics*
  • Steroid Hydroxylases / metabolism
  • Transfection
  • Zona Glomerulosa / enzymology*

Substances

  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Recombinant Proteins
  • Cytochrome P-450 Enzyme System
  • Steroid Hydroxylases
  • Cytochrome P-450 CYP11B2

Associated data

  • GENBANK/S63834
  • GENBANK/S63835
  • GENBANK/S63836
  • GENBANK/S63837
  • GENBANK/S63838
  • GENBANK/S64136
  • GENBANK/S64544
  • GENBANK/U11085
  • GENBANK/U11086
  • GENBANK/U11087