Abstract
Clones expressing a partial human cytomegalovirus putative fusion receptor were selected by binding specifically to monoclonal anti-idiotypic antibodies that mimic glycoprotein H. cDNA was isolated from 2 of the clones (131 and 611) and fused in frame with the glutathione S-transferase gene in a pGEX-4T-1 vector. Two purified peptides (FR131 and FR611) were produced: both were shown to bind specifically to the monoclonal anti-idiotypic antibodies and inhibit virus/cell fusion and viral plaque formation in a specific and dose-dependent manner. This is the first demonstration of cloned peptides encoding a putative cell membrane receptor that are able to block cytomegalovirus infection.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Antibodies, Anti-Idiotypic / pharmacology
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Antibodies, Monoclonal
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Blotting, Western
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Cell Fusion*
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Cells, Cultured
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Cloning, Molecular
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Cytomegalovirus / physiology*
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Gene Expression
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Glutathione Transferase / biosynthesis
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Humans
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Lung
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Membrane Proteins / drug effects
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Membrane Proteins / physiology
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Peptide Fragments / biosynthesis
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Peptide Fragments / pharmacology*
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Receptors, Virus / drug effects
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Receptors, Virus / physiology*
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / drug effects
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Recombinant Fusion Proteins / metabolism
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Viral Plaque Assay
Substances
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Antibodies, Anti-Idiotypic
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Antibodies, Monoclonal
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Membrane Proteins
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Peptide Fragments
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Receptors, Virus
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Recombinant Fusion Proteins
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cytomegalovirus receptor
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Glutathione Transferase