Nucleotide sequence and characterization of erythromycin resistance determinant that encodes macrolide 2'-phosphotransferase I in Escherichia coli

Antimicrob Agents Chemother. 1995 Oct;39(10):2359-63. doi: 10.1128/AAC.39.10.2359.

Abstract

The DNA fragment (3.3 kb) containing the erythromycin resistance determinant was cloned from Escherichia coli Tf481A and sequenced. Deletion and complementation analyses indicated that the expression of high-level resistance to erythromycin requires two genes, mphA and mrx, which encode macrolide 2'-phosphotransferase I and an unidentified hydrophobic protein, respectively.

MeSH terms

  • Amino Acid Sequence
  • Anti-Bacterial Agents / metabolism*
  • Anti-Bacterial Agents / pharmacology*
  • Base Sequence
  • Drug Resistance, Microbial / genetics*
  • Erythromycin / pharmacology*
  • Escherichia coli / enzymology*
  • Molecular Sequence Data
  • Open Reading Frames
  • Phosphotransferases (Alcohol Group Acceptor) / genetics*
  • Phosphotransferases / genetics*

Substances

  • Anti-Bacterial Agents
  • Erythromycin
  • Phosphotransferases
  • Phosphotransferases (Alcohol Group Acceptor)
  • macrolide 2'-kinase

Associated data

  • GENBANK/D16251