Abstract
The DNA fragment (3.3 kb) containing the erythromycin resistance determinant was cloned from Escherichia coli Tf481A and sequenced. Deletion and complementation analyses indicated that the expression of high-level resistance to erythromycin requires two genes, mphA and mrx, which encode macrolide 2'-phosphotransferase I and an unidentified hydrophobic protein, respectively.
MeSH terms
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Amino Acid Sequence
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Anti-Bacterial Agents / metabolism*
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Anti-Bacterial Agents / pharmacology*
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Base Sequence
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Drug Resistance, Microbial / genetics*
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Erythromycin / pharmacology*
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Escherichia coli / enzymology*
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Molecular Sequence Data
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Open Reading Frames
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Phosphotransferases (Alcohol Group Acceptor) / genetics*
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Phosphotransferases / genetics*
Substances
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Anti-Bacterial Agents
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Erythromycin
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Phosphotransferases
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Phosphotransferases (Alcohol Group Acceptor)
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macrolide 2'-kinase