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J Biol Chem. 1996 Mar 15;271(11):5961-4.

Dominant negative stat3 mutant inhibits interleukin-6-induced Jak-STAT signal transduction.

Author information

1
Laboratoires Glaxo-Wellcome, Centre de Recherche, ZA de Courtaboeuf, France.

Abstract

Interleukin-6 (IL-6) induces tyrosine phosphorylation and activation of the latent transcription factor Stat3 in HepG2 cells. Mutation of Stat3 tyrosine 705 to phenylalanine (Y705F) inhibits IL-6-induced tyrosine phosphorylation of this Stat3 mutant in transfected HepG2 cells. In cotransfections of HepG2 cells, the Stat3 mutant Y705F causes a reduction of the tyrosine phosphorylation of wild type Stat3-FLAG. Moreover, Y705F inhibits the action of endogenous Stat3 in cotransfected cells, reducing IL-6 induction of a Stat3-responsive reporter construct. Y705F therefore acts as a dominant negative mutation of Stat3.

PMID:
8626374
DOI:
10.1074/jbc.271.11.5961
[Indexed for MEDLINE]
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