Abstract
Transforming growth factor-beta (TGF-beta) regulates many aspects of cellular function. A member of the mitogen-activated protein kinase kinase kinase (MAPKKK) family, TAK1, was previously identified as a mediator in the signaling pathway of TGF-beta superfamily members. The yeast two-hybrid system has now revealed two human proteins, termed TAB1 and TAB2 (for TAK1 binding protein), that interact with TAK1. TAB1 and TAK1 were co-immunoprecipitated from mammalian cells. Overproduction of TAB1 enhanced activity of the plasminogen activator inhibitor 1 gene promoter, which is regulated by TGF-beta, and increased the kinase activity of TAK1. TAB1 may function as an activator of the TAK1 MAPKKK in TGF-beta signal transduction.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing*
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Amino Acid Sequence
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Animals
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Base Sequence
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Carrier Proteins / chemistry
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Carrier Proteins / metabolism*
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Cell Line
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Cloning, Molecular
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Enzyme Activation
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Genes, Reporter
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Humans
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Intracellular Signaling Peptides and Proteins*
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MAP Kinase Kinase Kinases*
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Mice
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Molecular Sequence Data
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Plasminogen Activator Inhibitor 1 / genetics
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Promoter Regions, Genetic
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Protein Serine-Threonine Kinases / metabolism*
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Signal Transduction*
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Transfection
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Transformation, Genetic
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Transforming Growth Factor beta / metabolism*
Substances
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Adaptor Proteins, Signal Transducing
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Carrier Proteins
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Intracellular Signaling Peptides and Proteins
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Plasminogen Activator Inhibitor 1
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Recombinant Fusion Proteins
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TAB1 protein, MAPKKK activator, vertebrate
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TAB1 protein, human
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Transforming Growth Factor beta
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Protein Serine-Threonine Kinases
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MAP Kinase Kinase Kinases
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MAP kinase kinase kinase 7