Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody (MabB23) specific for human plasma apolipoprotein B-100

Gene. 1996 Mar 9;169(2):237-9. doi: 10.1016/0378-1119(95)00807-1.

Abstract

We have determined the nucleotide sequences encoding the heavy and light chains of the Fab fragment of murine monoclonal antibody MabB23(gamma2b,lambda), which is specific for human plasma apolipoprotein B-100 of low-density lipoproteins. The sequence analyses revealed that the variable regions of the heavy and light chains are members of mouse heavy-chain subgroup I(B) and lambda light-chain, respectively. A few unusual amino acids in the framework and constant regions of the heavy-chain were also noticed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / genetics*
  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal, Murine-Derived
  • Apolipoprotein B-100
  • Apolipoproteins B / immunology*
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary
  • Humans
  • Immunoglobulin Fab Fragments / genetics*
  • Immunoglobulin Heavy Chains / chemistry
  • Immunoglobulin Heavy Chains / genetics*
  • Immunoglobulin Light Chains / chemistry
  • Immunoglobulin Light Chains / genetics*
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Sequence Analysis
  • Tumor Cells, Cultured

Substances

  • Antibodies, Monoclonal
  • Antibodies, Monoclonal, Murine-Derived
  • Apolipoprotein B-100
  • Apolipoproteins B
  • DNA, Complementary
  • Immunoglobulin Fab Fragments
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Light Chains
  • heavy chain, monoclonal antibody B23
  • light chain, monoclonal antibody B23

Associated data

  • GENBANK/U28967
  • GENBANK/U28970