Abstract
We have determined the nucleotide sequences encoding the heavy and light chains of the Fab fragment of murine monoclonal antibody MabB23(gamma2b,lambda), which is specific for human plasma apolipoprotein B-100 of low-density lipoproteins. The sequence analyses revealed that the variable regions of the heavy and light chains are members of mouse heavy-chain subgroup I(B) and lambda light-chain, respectively. A few unusual amino acids in the framework and constant regions of the heavy-chain were also noticed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / genetics*
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Antibodies, Monoclonal / immunology
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Antibodies, Monoclonal, Murine-Derived
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Apolipoprotein B-100
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Apolipoproteins B / immunology*
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Base Sequence
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Cloning, Molecular
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DNA, Complementary
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Humans
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Immunoglobulin Fab Fragments / genetics*
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Immunoglobulin Heavy Chains / chemistry
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Immunoglobulin Heavy Chains / genetics*
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Immunoglobulin Light Chains / chemistry
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Immunoglobulin Light Chains / genetics*
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Sequence Analysis
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Tumor Cells, Cultured
Substances
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Antibodies, Monoclonal
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Antibodies, Monoclonal, Murine-Derived
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Apolipoprotein B-100
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Apolipoproteins B
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DNA, Complementary
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Immunoglobulin Fab Fragments
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Immunoglobulin Heavy Chains
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Immunoglobulin Light Chains
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heavy chain, monoclonal antibody B23
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light chain, monoclonal antibody B23
Associated data
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GENBANK/U28967
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GENBANK/U28970