Abstract
The antimicrobial mechanism of totarol was studied using Pseudomonas aeruginosa IFO 3080. This diterpene inhibited oxygen consumption and respiratory-driven proton translocation in whole cells, and oxidation of NADH in membrane preparation. NADH-cytochrome c reductase was inhibited by totarol while cytochrome c oxidase was not. NADH-DPIP reductase and NADH-CoQ reductase were also inhibited. The site of respiratory inhibition of totarol was thought to be near CoQ in the bacterial electron transport chain.
MeSH terms
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Abietanes
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Anti-Bacterial Agents / pharmacology*
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Cell Membrane / metabolism
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Diterpenes / pharmacology*
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Electron Transport / drug effects
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Electron Transport Complex I
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Electron Transport Complex IV / metabolism
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Escherichia coli / drug effects
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Microbial Sensitivity Tests
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NAD / metabolism
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NADH Dehydrogenase / antagonists & inhibitors
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NADH, NADPH Oxidoreductases / antagonists & inhibitors
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NADH, NADPH Oxidoreductases / metabolism*
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Oxygen Consumption / drug effects
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Plant Extracts*
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Plant Roots
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Pseudomonas aeruginosa / drug effects*
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Pseudomonas aeruginosa / metabolism
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Staphylococcus aureus / drug effects
Substances
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Abietanes
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Anti-Bacterial Agents
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Diterpenes
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Plant Extracts
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NAD
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totarol
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NADH, NADPH Oxidoreductases
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NADH Dehydrogenase
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Electron Transport Complex IV
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Electron Transport Complex I