Decreased beta 2-adrenergic receptor-mRNA and loricrin-mRNA, and increased involucrin-mRNA transcripts in psoriatic epidermis: analysis by reverse transcription-polymerase chain reaction

Br J Dermatol. 1996 Jun;134(6):1065-9.

Abstract

Psoriatic hyperproliferative epidermis is characterized by a decreased beta 2-adrenergic adenylate cyclase response as well as by altered differentiation markers that include decreased loricrin and increased involucrin. Using a quantitative reverse transcription-polymerase chain reaction, we analysed the expression of beta 2-adrenergic receptor-mRNA, loricrin-mRNA, and involucrin-mRNA in the epidermis of five patients with psoriasis vulgaris. The mRNAs of the beta 2-adrenergic receptor and loricrin in the involved epidermis were significantly decreased, by 0.35-fold (P < 0.01) and 0.55-fold (P < 0.05) respectively, compared with uninvolved epidermis. In contrast, the involucrin mRNA expression of the involved epidermis was significantly increased, by 3.77-fold (P < 0.01). No significant difference in beta-actin mRNA transcripts was detected between the involved and the uninvolved epidermis. These results indicate that the altered expression of the beta 2-adrenergic receptor, loricrin, and involucrin, in the psoriatic involved epidermis, is associated with different amounts of each mRNA transcripts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / genetics
  • Actins / metabolism
  • Adult
  • Base Sequence
  • Epidermis / metabolism*
  • Female
  • Humans
  • Male
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Middle Aged
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Protein Precursors / genetics
  • Protein Precursors / metabolism*
  • Psoriasis / metabolism*
  • RNA, Messenger / genetics
  • Receptors, Adrenergic, beta-2 / genetics
  • Receptors, Adrenergic, beta-2 / metabolism*

Substances

  • Actins
  • Membrane Proteins
  • Protein Precursors
  • RNA, Messenger
  • Receptors, Adrenergic, beta-2
  • loricrin
  • involucrin