Abstract
To elucidate the genetic basis of salt-sensitivity in mammalian hypertension, we isolated six rat complementary DNAs by functional complementation in yeast. These genes were able to substitute for the salt-tolerant activity of HALI which confers salt tolerance by modulating the cation transport system in yeast. We identified these genes as beta-globin, lambda-crystallin, androgen-regulated protein, mitochondrial cytochrome b, a homologue of infant brain cDNA, and a novel gene, called salt-tolerant protein (STP). STP contains 1964 bp nucleotides and an open reading frame which encodes 496 amino acid residues. Northern blot analysis showed that STP mRNA is expressed in various rat tissues.
MeSH terms
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ATPases Associated with Diverse Cellular Activities
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Adaptor Proteins, Signal Transducing*
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Amino Acid Sequence
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Animals
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Base Sequence
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Carrier Proteins*
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DNA, Complementary / genetics
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Drug Resistance
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Fungal Proteins / genetics*
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Gene Library
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Genetic Complementation Test
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Hypertension / etiology
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Intracellular Signaling Peptides and Proteins
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Kidney / chemistry
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LIM Domain Proteins
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Microtubule-Associated Proteins
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Minor Histocompatibility Antigens
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Molecular Sequence Data
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Proteasome Endopeptidase Complex
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Rats
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Rats, Wistar
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Saccharomyces cerevisiae / drug effects
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins*
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Sodium Chloride / pharmacology*
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Tissue Distribution
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Transcription Factors / genetics
Substances
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Adaptor Proteins, Signal Transducing
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Carrier Proteins
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DNA, Complementary
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Fungal Proteins
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HAL1 protein, S cerevisiae
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Intracellular Signaling Peptides and Proteins
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LIM Domain Proteins
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Microtubule-Associated Proteins
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Minor Histocompatibility Antigens
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PSMC5 protein, human
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Trip10 protein, rat
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Sodium Chloride
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Proteasome Endopeptidase Complex
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ATPases Associated with Diverse Cellular Activities