Abstract
Posttranscriptional components of the gene expression mechanism of rice tungro bacilliform virus (RTBV) were studied in transiently transfected protoplasts. RTBV translates several open reading frames from a polycistronic mRNA by leaky scanning. This mechanism is supported by the particular sequence features of the corresponding genome region and does not require a virus-encoded transactivator.
MeSH terms
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Badnavirus / genetics*
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Badnavirus / metabolism
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Chloramphenicol O-Acetyltransferase
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Genes, Plant*
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Genome, Viral
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Open Reading Frames*
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Oryza / virology*
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Promoter Regions, Genetic
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Protein Biosynthesis*
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RNA, Messenger / biosynthesis
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RNA, Viral / metabolism*
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Recombinant Fusion Proteins / biosynthesis
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Transcription, Genetic
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Transfection
Substances
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RNA, Messenger
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RNA, Viral
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Recombinant Fusion Proteins
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Chloramphenicol O-Acetyltransferase