Complete nucleotide sequence of Colorado tick fever virus segments M6, S1 and S2

J Gen Virol. 1997 Nov:78 ( Pt 11):2895-9. doi: 10.1099/0022-1317-78-11-2895.

Abstract

The nucleotide sequences of the tenth (M6), eleventh (S1) and twelfth (S2) dsRNA genomic segments of the Florio strain (N-7180) of Colorado tick fever virus were determined and found to be 675, 998 and 1884 bp, respectively, in length. A nonanucleotide motif and a hexanucleotide motif were found to be highly conserved in the 5' and 3' non-coding regions (NCRs), respectively, of the three segments. The first and last three nucleotides of each segment were of inverted complementarity, and segment-specific inverted terminal repeats were detected in the NCRs of the three segments. These findings suggest the occurrence of intracellular panhandle structures for the RNA transcripts. A readthrough phenomenon is suspected in segment M6. The environment surrounding the opal codon (position 1052-1054) of segment M6 conforms to that of leaky opal codons described in the literature.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Colorado tick fever virus / genetics*
  • Genome, Viral*
  • Molecular Sequence Data
  • RNA, Viral / genetics*
  • Repetitive Sequences, Nucleic Acid
  • Sequence Analysis

Substances

  • RNA, Viral

Associated data

  • GENBANK/AF000720
  • GENBANK/U53227
  • GENBANK/U72694