Abstract
Light-regulated translation of chloroplast messenger RNAs (mRNAs) requires trans-acting factors that interact with the 5' untranslated region (UTR) of these mRNAs. Chloroplast polyadenylate-binding protein (cPABP) specifically binds to the 5'-UTR of the psbA mRNA and is essential for translation of this mRNA. A protein disulfide isomerase that is localized to the chloroplast and copurifies with cPABP was shown to modulate the binding of cPABP to the 5'-UTR of the psbA mRNA by reversibly changing the redox status of cPABP through redox potential or adenosine 5'-diphosphate-dependent phosphorylation. This mechanism allows for a simple reversible switch regulating gene expression in the chloroplast.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Diphosphate / metabolism
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Amino Acid Sequence
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Animals
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Binding Sites
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Catalysis
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Chlamydomonas reinhardtii / enzymology
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Chlamydomonas reinhardtii / genetics*
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Chlamydomonas reinhardtii / metabolism
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Chloroplasts / genetics*
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Chloroplasts / metabolism
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Cloning, Molecular
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Dithiothreitol / pharmacology
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Gene Expression Regulation*
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Glutathione Disulfide / pharmacology
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Molecular Sequence Data
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Oxidation-Reduction
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Phosphorylation
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Photosynthetic Reaction Center Complex Proteins / genetics
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Photosystem II Protein Complex
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Protein Biosynthesis*
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Protein Disulfide-Isomerases / chemistry
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Protein Disulfide-Isomerases / genetics
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Protein Disulfide-Isomerases / metabolism*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA-Binding Proteins / metabolism*
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Recombinant Fusion Proteins / metabolism
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Sequence Homology, Amino Acid
Substances
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Photosynthetic Reaction Center Complex Proteins
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Photosystem II Protein Complex
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RNA, Messenger
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RNA-Binding Proteins
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Recombinant Fusion Proteins
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Adenosine Diphosphate
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Protein Disulfide-Isomerases
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Dithiothreitol
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Glutathione Disulfide