Abstract
Using the yeast two-hybrid system, we isolated a cDNA encoding a novel human protein, named Pir51, that strongly interacts with human Rad51 recombinase. Analysis in vitro confirmed the interaction between Rad51 and Pir51. Pir51 mRNA is expressed in a number of human organs, most notably in testis, thymus, colon and small intestine. The Pir51 gene locus was mapped to chromosome 12p13.1-13. 2 by fluorescence in situ hybridization. The Pir51 protein was expressed in Escherichia coli and purified to near homogeneity. Biochemical analysis shows that the Pir51 protein binds both single- and double-stranded DNA, and is capable of aggregating DNA. The protein also binds RNA. The Pir51 protein may represent a new member of the multiprotein complexes postulated to carry out homologous recombination and DNA repair in mammalian cells.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Chromosomes, Human, Pair 12 / genetics
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DNA / metabolism
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DNA, Complementary / genetics
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / isolation & purification
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DNA-Binding Proteins / metabolism*
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Genes*
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HeLa Cells
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Humans
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In Situ Hybridization, Fluorescence
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Molecular Sequence Data
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Organ Specificity
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Protein Binding
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RNA / metabolism
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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RNA-Binding Proteins
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Rad51 Recombinase
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Recombination, Genetic
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Saccharomyces cerevisiae
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Saccharomyces cerevisiae Proteins
Substances
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DNA, Complementary
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DNA-Binding Proteins
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RAD51AP1 protein, human
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RNA, Messenger
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RNA-Binding Proteins
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Saccharomyces cerevisiae Proteins
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RNA
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DNA
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RAD51 protein, S cerevisiae
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RAD51 protein, human
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Rad51 Recombinase