Beet soil-borne virus RNA 1: genetic analysis enabled by a starting sequence generated with primers to highly conserved helicase-encoding domains

J Gen Virol. 1997 Dec:78 ( Pt 12):3161-5. doi: 10.1099/0022-1317-78-12-3161.

Abstract

The complete sequence of the 5834 nucleotides of RNA 1 of beet soil-borne furovirus (BSBV, Ahlum isolate) was determined using a PCR product obtained with primers to highly conserved coding regions for helicase-like proteins in RNA 1 of furo-, hordei- and tobraviruses as a starting sequence. Unknown parts of the sequence upstream and downstream of this starting sequence were amplified by means of RT-PCR techniques using combinations of specific and random primers. BSBV RNA 1 contains one large ORF for a readthrough protein with a molecular mass of 204 kDa (204K protein) which is interrupted internally by a UAA stop codon terminating the coding region for a protein of 145 kDa (145K protein). The N- and C-terminal parts of the 145K protein and the readthrough domain of the 204K protein contain methyltransferase, helicase and RNA-dependent RNA polymerase motifs, respectively. Unlike other furo- and tobraviruses BSBV contains no further genes on its RNA 1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chenopodiaceae / virology*
  • Conserved Sequence
  • DNA Helicases / genetics*
  • Molecular Sequence Data
  • Plant Viruses / genetics*
  • RNA / genetics
  • RNA, Viral / genetics*
  • Sequence Alignment
  • Sequence Analysis*

Substances

  • RNA primers
  • RNA, Viral
  • RNA
  • DNA Helicases

Associated data

  • GENBANK/Z97873