Abstract
A cDNA for mouse prolyl endopeptidase (PEP) was cloned and its nucleotide sequence determined. The overall amino acid sequence identity between mouse and other mammalian PEPs was about 96%. A specific inhibitor of PEP, N-benzyloxycarbonyl-thioprolyl-thioprolinal- dimethylacetal (ZTTA), inhibited DNA synthesis by Swiss 3T3 cells. Mouse PEP was shown to be localized partly in restricted nuclear regions. These results suggest that PEP participates in mammalian DNA synthesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells / drug effects
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3T3 Cells / enzymology*
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Southern
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Cell Division / drug effects
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Cell Nucleus / enzymology
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Cloning, Molecular
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Cytoplasm / enzymology
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DNA / biosynthesis*
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Fluorescent Antibody Technique, Indirect
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Immunoblotting
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Mice
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Molecular Sequence Data
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Prolyl Oligopeptidases
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Serine Endopeptidases / genetics*
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Serine Endopeptidases / immunology
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Serine Endopeptidases / metabolism*
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Thiazoles / pharmacology
Substances
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N-benzyloxycarbonyl-thioprolyl-thioprolinal-dimethylacetal
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Thiazoles
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DNA
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Serine Endopeptidases
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Prolyl Oligopeptidases