Abstract
Cdc45p, a protein essential for initiation of DNA replication, associates with chromatin after "start" in late G1 and during the S phase of the cell cycle. Binding of Cdc45p to chromatin depends on Clb-Cdc28 kinase activity as well as functional Cdc6p and Mcm2p, which suggests that Cdc45p associates with the prereplication complex after activation of S-phase cyclin-dependent kinases (CDKs). As indicated by the timing and the CDK dependence, binding of Cdc45p to chromatin is crucial for commitment to initiation of DNA replication. During S phase, Cdc45p physically interacts with minichromosome maintenance (MCM) proteins on chromatin; however, dissociation of Cdc45p from chromatin is slower than that of MCMs, which indicates that the proteins are released by different mechanisms.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
CDC28 Protein Kinase, S cerevisiae / metabolism
-
Carrier Proteins / metabolism*
-
Cell Cycle Proteins / metabolism
-
Chromatin / metabolism*
-
Chromosomal Proteins, Non-Histone
-
Cyclin-Dependent Kinases / metabolism*
-
Cyclins / metabolism
-
DNA Replication*
-
DNA, Fungal / biosynthesis
-
DNA-Binding Proteins / metabolism
-
Enzyme Activation
-
Fungal Proteins / metabolism
-
G1 Phase
-
Nuclear Proteins / metabolism*
-
Origin Recognition Complex
-
S Phase*
-
Saccharomyces cerevisiae / cytology
-
Saccharomyces cerevisiae / metabolism*
-
Saccharomyces cerevisiae Proteins*
Substances
-
CDC45 protein, S cerevisiae
-
CDC6 protein, S cerevisiae
-
Carrier Proteins
-
Cell Cycle Proteins
-
Chromatin
-
Chromosomal Proteins, Non-Histone
-
Cyclins
-
DNA, Fungal
-
DNA-Binding Proteins
-
Fungal Proteins
-
Nuclear Proteins
-
Origin Recognition Complex
-
Saccharomyces cerevisiae Proteins
-
CDC28 Protein Kinase, S cerevisiae
-
Cyclin-Dependent Kinases
-
MCM2 protein, S cerevisiae