Abstract
Twenty feline neoplasms were sequenced in the region from exons 5 to 8 for the presence of tumour suppressor gene p53 mutations. In a spindle cell sarcoma of the bladder, a missense mutation (codon 164 AAG-->GAG, lysine-->glutamic acid) in exon 5 was detected. In a pleomorphic sarcoma, a 23 bp deletion involving the splicing junction between intron 5 and exon 6 was observed. In a fibrosarcoma, a 6 bp deletion of p53 covering 2 bp of exon 7 and 4 bp of intron 7, including the splicing junction, was found. The study demonstrates three new p53 mutations in different types of sarcomas in cats.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cat Diseases / genetics*
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Cat Diseases / pathology
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Cats
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DNA, Neoplasm / chemistry
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Female
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Fibrosarcoma / genetics
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Fibrosarcoma / pathology
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Fibrosarcoma / veterinary
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Gene Deletion
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Genes, p53 / genetics*
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Male
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Molecular Sequence Data
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Mutation / genetics
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Polymerase Chain Reaction / veterinary
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Sarcoma / genetics
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Sarcoma / pathology
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Sarcoma / veterinary*
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Sequence Analysis, DNA
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Skin Neoplasms / genetics
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Skin Neoplasms / pathology
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Skin Neoplasms / veterinary*
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Urinary Bladder Neoplasms / genetics
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Urinary Bladder Neoplasms / pathology
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Urinary Bladder Neoplasms / veterinary*