Abstract
Four strains of Butyrivibrio fibrisolvens, transformed with a gene encoding fluoroacetate dehalogenase, maintained a combined population of 10(6) to 10(7) cells ml-1 in the rumens of test sheep. Five inoculated sheep showed markedly reduced toxicological symptoms after fluoroacetate poisoning when behavioral, physiological, and histological effects were compared with those of five uninoculated control sheep.
MeSH terms
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Animals
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Fluoroacetates / poisoning*
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Gram-Negative Anaerobic Straight, Curved, and Helical Rods / enzymology
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Gram-Negative Anaerobic Straight, Curved, and Helical Rods / genetics*
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Hydrolases / genetics*
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Hydrolases / metabolism
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Plasmids
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Poisoning / prevention & control
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Poisoning / veterinary
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Recombinant Proteins / metabolism
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Rumen / microbiology*
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Sheep
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Sheep Diseases / chemically induced*
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Sheep Diseases / prevention & control
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Transformation, Bacterial
Substances
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Fluoroacetates
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Recombinant Proteins
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fluoroacetic acid
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Hydrolases
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fluoroacetate-specific dehalogenase