Abstract
Gene specific PCR primers were constructed for five mouse and three bovine CXC chemokine genes. The mouse genes were assigned using SSCP analyses of the Jackson BSS backcross panel to two groups on chromosome 5. One group containing Gro1 and Mip2 cosegregated with reference markers Alb1 and Btc, and was positioned 2.2 cM proximal to a group comprising Ifi10, Mig, and Scyb5. The bovine genes IL8, GRO1, and GRO3, mapped using bovine x hamster somatic cell hybrids, were all found to be located on chromosome 6. The locations of these genes in these two animal species are consistent with the positions in humans (4q13-->q21), and previous syntenic relationships among these three mammals.
MeSH terms
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Animals
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Cattle / genetics*
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Chemokine CXCL1
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Chemokine CXCL2
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Chemokine CXCL9
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Chemokines*
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Chemokines, CXC / genetics*
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Chemotactic Factors / genetics
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Chromosome Mapping*
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Chromosomes, Human, Pair 4
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Cricetinae
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Crosses, Genetic
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DNA Primers
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Genetic Markers
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Growth Substances / genetics
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Humans
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Hybrid Cells
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Intercellular Signaling Peptides and Proteins*
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Interleukin-8 / genetics
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Mice
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Mice, Inbred C57BL / genetics*
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Monokines / genetics
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Muridae / genetics*
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Polymerase Chain Reaction
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Polymorphism, Single-Stranded Conformational
Substances
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CXCL1 protein, Bos taurus
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CXCL1 protein, human
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CXCL3 protein, human
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CXCL9 protein, human
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Chemokine CXCL1
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Chemokine CXCL2
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Chemokine CXCL9
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Chemokines
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Chemokines, CXC
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Chemotactic Factors
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Cxcl1 protein, mouse
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DNA Primers
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Genetic Markers
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Growth Substances
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Intercellular Signaling Peptides and Proteins
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Interleukin-8
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Monokines