Abstract
Pseudomonas aeruginosa nalB mutants which hyperexpress the MexAB-OprM multidrug efflux system produce reduced levels of several extracellular virulence factors known to be regulated by quorum sensing. Such mutants also produce less acylated homoserine lactone autoinducer PAI-1, consistent with an observed reduction in lasI expression. These data suggest that PAI-1 is a substrate for MexAB-OprM, and its resulting exclusion from cells hyperexpressing MexAB-OprM limits PAI-1-dependent activation of lasI and the virulence genes.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
4-Butyrolactone / analogs & derivatives
-
4-Butyrolactone / biosynthesis
-
Bacterial Proteins / biosynthesis
-
Bacterial Proteins / metabolism*
-
Biological Transport
-
Drug Resistance, Multiple / genetics
-
Gene Expression Regulation, Bacterial
-
Pheromones / biosynthesis
-
Pseudomonas aeruginosa / pathogenicity
-
Pseudomonas aeruginosa / physiology*
-
Pyocyanine / biosynthesis
-
Signal Transduction / genetics
-
Virulence / genetics
Substances
-
Bacterial Proteins
-
LasI protein, Pseudomonas aeruginosa
-
Pheromones
-
N-(3-oxohexanoyl)-3-aminodihydro-2(3H)-furanone
-
Pyocyanine
-
4-Butyrolactone