Murine DEP-1, a receptor protein tyrosine phosphatase, is expressed in macrophages and is regulated by CSF-1 and LPS

J Leukoc Biol. 1998 Nov;64(5):692-701. doi: 10.1002/jlb.64.5.692.

Abstract

The spectrum of protein tyrosine phosphatases (PTPs) expressed in bone marrow-derived murine macrophages (BMMs) was examined using reverse transcriptase-polymerase chain reaction. Ten different PTP cDNAs were isolated and in this study we focus on mDEP-1, a type III receptor PTP. Three mDEP-1 transcripts were expressed in primary macrophages and macrophage cell lines and were induced during macrophage differentiation of M1 myeloid leukemia cells. A variant mRNA was identified that encodes an alternate carboxyl-terminus and 3' UTR. The expression of mDEP-1 was down-regulated by CSF-1 (macrophage colony-stimulating factor) and up-regulated by bacterial lipopolysaccharide, an important physiological regulator of macrophage function that opposes CSF-1 action. Whole mount in situ hybridization, and immunolocalization of the protein, confirmed that mDEP-1 is expressed by a subset of embryonic macrophages in the liver and mesenchyme. mDEP-1 was also detected in the eye and peripheral nervous system of the developing embryo. Attempts to express mDEP-1 constitutively in the macrophage cell line RAW264 were unsuccessful, with results suggesting that the gene product inhibits cell proliferation.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Bone Marrow Cells / metabolism*
  • Cell Differentiation
  • Cell Division
  • Cell Lineage
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Eye / embryology
  • Eye / metabolism
  • Eye Proteins / biosynthesis
  • Eye Proteins / genetics
  • Fluorescent Antibody Technique, Indirect
  • Gene Expression Regulation, Developmental / drug effects*
  • In Situ Hybridization
  • Lipopolysaccharides / pharmacology*
  • Macrophage Colony-Stimulating Factor / physiology*
  • Macrophages / classification
  • Macrophages / metabolism*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Nerve Tissue Proteins / biosynthesis
  • Nerve Tissue Proteins / genetics
  • Peripheral Nerves / embryology
  • Peripheral Nerves / metabolism
  • Polymerase Chain Reaction
  • Protein Tyrosine Phosphatases / biosynthesis*
  • Protein Tyrosine Phosphatases / genetics
  • Protein Tyrosine Phosphatases / physiology
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Receptor-Like Protein Tyrosine Phosphatases, Class 3

Substances

  • DNA, Complementary
  • Eye Proteins
  • Lipopolysaccharides
  • Nerve Tissue Proteins
  • RNA, Messenger
  • Macrophage Colony-Stimulating Factor
  • Protein Tyrosine Phosphatases
  • Ptprj protein, mouse
  • Receptor-Like Protein Tyrosine Phosphatases, Class 3