New type of Sendai virus vector provides transgene-free iPS cells derived from chimpanzee blood

Yasumitsu Fujie; Noemi Fusaki; Tomohiko Katayama; Makoto Hamasaki; Yumi Soejima; Minami Soga; Hiroshi Ban; Mamoru Hasegawa; Satoshi Yamashita; Shigemi Kimura; Saori Suzuki; Tetsuro Matsuzawa; Hirofumi Akari; Takumi Era

doi:10.1371/journal.pone.0113052

New type of Sendai virus vector provides transgene-free iPS cells derived from chimpanzee blood

PLoS One. 2014 Dec 5;9(12):e113052. doi: 10.1371/journal.pone.0113052. eCollection 2014.

Authors

Affiliations

¹ Department of Cell Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan.
² DNAVEC Corporation, 6 Ookubo, Tsukuba, Ibaragi 300-2611, Japan; Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama, Japan.
³ DNAVEC Corporation, 6 Ookubo, Tsukuba, Ibaragi 300-2611, Japan.
⁴ Department of Neurology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-8556, Japan.
⁵ Department of Child Development, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-8556, Japan.
⁶ Section of Comparative Microbiology and Immunology, Center for Human Evolution Modeling Research, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan.
⁷ Section of Language and Intelligence, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan.
⁸ Section of Comparative Microbiology and Immunology, Center for Human Evolution Modeling Research, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan; Laboratory of Evolutional Virology, Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Kyoto, 606-8507, Japan.

Abstract

Induced pluripotent stem cells (iPSCs) are potentially valuable cell sources for disease models and future therapeutic applications; however, inefficient generation and the presence of integrated transgenes remain as problems limiting their current use. Here, we developed a new Sendai virus vector, TS12KOS, which has improved efficiency, does not integrate into the cellular DNA, and can be easily eliminated. TS12KOS carries KLF4, OCT3/4, and SOX2 in a single vector and can easily generate iPSCs from human blood cells. Using TS12KOS, we established iPSC lines from chimpanzee blood, and used DNA array analysis to show that the global gene-expression pattern of chimpanzee iPSCs is similar to those of human embryonic stem cell and iPSC lines. These results demonstrated that our new vector is useful for generating iPSCs from the blood cells of both human and chimpanzee. In addition, the chimpanzee iPSCs are expected to facilitate unique studies into human physiology and disease.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation / genetics*
Cell Line
Cellular Reprogramming / genetics
Gene Expression
Genetic Vectors*
Humans
Induced Pluripotent Stem Cells / cytology
Kruppel-Like Factor 4
Pan troglodytes
Sendai virus / genetics*
Transduction, Genetic*
Transgenes

Grants and funding

This study was supported in part by grants from the Ministry of Health, Labor, and Welfare of Japan, Precursory Research for Embryonic Science and Technology (PRESTO), and Core Research for Evolutional Science and Technology (CREST), Japan Science and Project of the Primate Research Institute, Kyoto University. DNAVEC Corporation provided support in the form of salaries for authors MH & HB, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. NF was provided with salary by JST grant (PRESTO). The specific roles of these authors are articulated in the 'author contributions' section.