LncRNA and mRNA expression profiles in brown adipose tissue of obesity-prone and obesity-resistant mice

Congcong Wang; Meng Duan; Jinhua Lin; Guowei Wang; He Gao; Mengsha Yan; Lin Chen; Jialing He; Wei Liu; Fei Yang; Shankuan Zhu

doi:10.1016/j.isci.2022.104809

LncRNA and mRNA expression profiles in brown adipose tissue of obesity-prone and obesity-resistant mice

iScience. 2022 Jul 20;25(8):104809. doi: 10.1016/j.isci.2022.104809. eCollection 2022 Aug 19.

Authors

Congcong Wang^{1

2}, Meng Duan^{1

2}, Jinhua Lin^{1

2}, Guowei Wang^{1

2}, He Gao^{1

2}, Mengsha Yan^{1

2}, Lin Chen^{1

2}, Jialing He^{1

2}, Wei Liu³, Fei Yang^{1

2}, Shankuan Zhu^{1

2}

Affiliations

¹ Chronic Disease Research Institute, The Children's Hospital, and National Clinical Research Center for Child Health, School of Public Health, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, China.
² Department of Nutrition and Food Hygiene, School of Public Health, School of Medicine, Zhejiang University, 866 Yu-hang-tang Road, Hangzhou, Zhejiang 310058, China.
³ Department of Biochemistry, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, China.

Abstract

Obesity-prone or obesity-resistant phenotypes can exist in individuals who consume the same diet type. Brown adipose tissue functions to dissipate energy in response to cold exposure or overfeeding. Long noncoding RNAs play important roles in a wide range of biological processes. However, systematic examination of lncRNAs in phenotypically divergent mice has not yet been reported. Here, the lncRNA expression profiles in BAT of HFD-induced C57BL/6J mice were investigated by high-throughput RNA sequencing. Genes that play roles in thermogenesis and related pathways were identified. We found lncRNA (Gm44502) may play a thermogenic role in obesity resistance by interacting with six mRNAs. Our results also indicated that seven differentially expressed lncRNAs (4930528G23Rik, Gm39490, Gm5627, Gm15551, Gm16083, Gm36860, Gm42002) may play roles in reducing heat production in obesity susceptibility by interacting with seven differentially expressed mRNAs. The screened lncRNAs may participate in the pathogenesis of weight regulation and provide insight into obesity therapy.

Keywords: Omics; Physiology; Transcriptomics.