Abstract
We identified and characterized two proteins that bind the promoter of surfactant protein B (SP-B) and affect its expression. Proteins A2 and B were identified and their cDNAs cloned and sequenced. Both were novel. They bound a 212-bp functional promoter region at an NF1 site, located between -184 and -198. Effects of these DNAbp on SP-B promoter activity were studied by contransfecting a reporter construct of this 212-bp sequence + luciferase, together with expression constructs for A2 and B into H441 cells. Alone, A2 and B expression elicited modest but statistically significant increases in SP-B promoter activity. When dexamethasone was added, B further increased SP-B promoter activity. For SP-B, basal expression and glucocorticoid responsiveness may involve a number of hitherto unknown gene activators.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Amino Acid Sequence
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Animals
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Cell Line
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Cloning, Molecular
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DNA, Complementary
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DNA-Binding Proteins / isolation & purification
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DNA-Binding Proteins / metabolism*
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Gene Amplification*
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Gene Expression*
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Gene Library
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Humans
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Lung / metabolism*
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Molecular Sequence Data
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Open Reading Frames
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Promoter Regions, Genetic*
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Proteolipids / biosynthesis*
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Proteolipids / chemistry
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Proteolipids / genetics
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Pulmonary Surfactant-Associated Proteins
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Pulmonary Surfactants / biosynthesis*
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Pulmonary Surfactants / chemistry
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Pulmonary Surfactants / genetics
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Rabbits
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Receptors, Cell Surface / chemistry
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Restriction Mapping
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Sequence Homology, Amino Acid
Substances
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DNA, Complementary
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DNA-Binding Proteins
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Proteolipids
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Pulmonary Surfactant-Associated Proteins
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Pulmonary Surfactants
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Receptors, Cell Surface
Associated data
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GENBANK/L10403
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GENBANK/L10404
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GENBANK/L10405