Abstract
We report the molecular cloning of a human cDNA that encodes a molecule having striking homology with Ras-specific GTPase-activating proteins (RasGAPs). Among previously described RasGAPs, the cDNA product is most closely related to Caenorhabditis elegans GAP-2, including a predicted coiled-coil structure near the carboxyl terminus. Expression of the cDNA in Saccharomyces cerevisiae defective in one of two RasGAPs, Ira2, complemented loss of the Ira2 function, indicating that the cDNA product functions as a RasGAP. The RasGAP-like gene is located on the human chromosome 1q25, the locus that appears to contain a hereditary prostate cancer susceptible gene, HPC1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Chromosome Mapping
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Chromosomes, Human, Pair 1*
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Consensus Sequence
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DNA, Complementary
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GTPase-Activating Proteins
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Genetic Predisposition to Disease / genetics*
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Humans
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Male
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Molecular Sequence Data
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Prostatic Neoplasms / genetics*
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Proteins / chemistry
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Proteins / genetics*
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Sequence Alignment
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Sequence Homology, Amino Acid
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ras GTPase-Activating Proteins
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ras Proteins / chemistry
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ras Proteins / genetics*
Substances
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DNA, Complementary
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GTPase-Activating Proteins
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Proteins
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Recombinant Proteins
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ras GTPase-Activating Proteins
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ras Proteins