LPS induces stefin A3 expression in mouse primary cultured glial cells

Toru Hosoi; Sawako Suzuki; Yasunobu Okuma; Akiko Kawakami; Naoki Ogawa; Koichiro Ozawa; Yasuyuki Nomura

doi:10.1016/j.molbrainres.2005.07.007

LPS induces stefin A3 expression in mouse primary cultured glial cells

Brain Res Mol Brain Res. 2005 Oct 31;140(1-2):138-41. doi: 10.1016/j.molbrainres.2005.07.007. Epub 2005 Sep 6.

Authors

Toru Hosoi¹, Sawako Suzuki, Yasunobu Okuma, Akiko Kawakami, Naoki Ogawa, Koichiro Ozawa, Yasuyuki Nomura

Affiliation

¹ Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.

PMID: 16143425
DOI: 10.1016/j.molbrainres.2005.07.007

Abstract

We searched for a gene that is up-regulated in response to LPS at a later time point in primary cultured glial cells. Using a Gene Chip Probe Array, we identified stefin A3, which is known as a cysteine protease inhibitor. As assessed by RT-PCR, we observed a time-dependent (2 to 48 h) up-regulation of stefin A3. The results indicate that stefin A3 is involved in infection and inflammation at a later time point.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Cells, Cultured
Cystatin A
Cystatin B
Cystatins / genetics*
DNA Primers
Gene Expression Regulation / drug effects*
Lipopolysaccharides / pharmacology*
Mice
Mice, Inbred C57BL
Neuroglia / cytology
Neuroglia / physiology*
Oligonucleotide Array Sequence Analysis

Substances

Cstb protein, mouse
Cystatin A
Cystatins
DNA Primers
Lipopolysaccharides
Stfa3 protein, mouse
Cystatin B