Abstract
ESCRT-II, a complex that sorts ubiquitinated membrane proteins to lysosomes, localizes to endosomes through interaction between the Vps36 subunit's GLUE domain and phosphatidylinositides (PIs). In yeast, a ubiquitin (Ub)-interacting NZF domain is inserted in Vps36 GLUE, whereas its mammalian counterpart, Eap45 GLUE, lacks the NZF domain. In the Eap45 GLUE-Ub complex structure, Ub binds far from the proposed PI-binding site of Eap45 GLUE, suggesting their independent binding.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Motifs
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Amino Acid Sequence
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Animals
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Binding Sites
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Carrier Proteins / chemistry*
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Carrier Proteins / metabolism*
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Crystallography, X-Ray
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Endosomal Sorting Complexes Required for Transport
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Endosomes / metabolism
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Mice
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Models, Molecular
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Molecular Sequence Data
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Protein Structure, Tertiary
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Protein Transport
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Saccharomyces cerevisiae Proteins / chemistry
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Sequence Alignment
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Transport Vesicles / metabolism*
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Ubiquitin / chemistry
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Ubiquitin / metabolism*
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Vesicular Transport Proteins / chemistry
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Vesicular Transport Proteins / metabolism
Substances
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Carrier Proteins
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EAP45 protein, mouse
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Endosomal Sorting Complexes Required for Transport
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Saccharomyces cerevisiae Proteins
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Ubiquitin
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Vesicular Transport Proteins
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Vps36 protein, S cerevisiae