The vertebrate DMRT gene family encodes putative transcription factors related to the sexual regulators Doublesex (Drosophila melanogaster) and MAB-3 (Caenorhabditis elegans). They share a highly conserved DNA binding motif, the DM domain. In human and mouse seven DMRT genes (DMRT1-DMRT7) have been analyzed. DMRT8, a gene related to DMRT7, is located on the X chromosome in placental mammals. While DMRT8 is single copy in most mammals, three copies are present in mouse, rat, and rabbit. Despite the loss of the DM domain, DMRT8 genes have been maintained in the mammalian lineage, suggesting a DM domain-independent function. In adult mouse, two Dmrt8 genes are expressed exclusively in testis. Dmrt8.1 mRNA was detected in Sertoli cells by in situ hybridization. In embryos, Dmrt8.2 shows a dynamic expression restricted to male and female gonads and might therefore be involved in sexual development in the mouse.