Abstract
The enzyme glutaminase in brain is responsible for the synthesis of neurotransmitter glutamate. We used the two-hybrid genetic selection system in yeast to look for interactors of glutaminase in human brain. We have identified two proteins containing PDZ domains, alpha1-syntrophin and a glutaminase-interacting protein, named GIP, that showed association with human glutaminase L, as deduced from specificity test of the two-hybrid system. The complete GIP cDNA clone has 1315 nucleotides with a 372-base open reading frame encoding a 124-amino acids protein. Glutaminase associates with both PDZ proteins through its C-terminal end; mutagenesis of single amino acids revealed the sequence -ESXV as essential for the interaction. These data suggest the possibility that PDZ domain-containing proteins are involved in the regulation of glutaminase in brain.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Binding Sites
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Brain / metabolism
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Calcium-Binding Proteins
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Glutaminase / chemistry*
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Glutaminase / genetics
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Glutaminase / metabolism*
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Humans
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Intracellular Signaling Peptides and Proteins*
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Isoenzymes / chemistry
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Isoenzymes / genetics
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Isoenzymes / metabolism
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Membrane Proteins / chemistry*
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Molecular Sequence Data
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Muscle Proteins / chemistry*
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Muscle Proteins / genetics
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Muscle Proteins / metabolism*
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Mutation / genetics
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Neurotransmitter Agents*
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Protein Binding
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Protein Structure, Tertiary
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Sequence Alignment
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Two-Hybrid System Techniques
Substances
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Calcium-Binding Proteins
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Intracellular Signaling Peptides and Proteins
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Isoenzymes
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Membrane Proteins
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Muscle Proteins
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Neurotransmitter Agents
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TAX1BP3 protein, human
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syntrophin alpha1
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Glutaminase