Abstract
The murine retrovirus SL3-3 is a potent inducer of T-cell lymphomas when inoculated into susceptible newborn mice. The proviral integration site sequences were surveyed in tumor DNAs by a simple two-step PCR method. From 20 SL3-3-induced tumors a total of 39 provirus-host junctions were amplified and sequenced. Seven showed homology to known sequences. These included the known common integration site c-myc as well as genes not previously identified as targets of provirus integration, namely N-ras and the genes coding for major histocompatibility complex class 11 E-beta, protein kinase C-eta, and T-cell receptor beta-chain. Among these genes, the integrations in c-myc as well as the one in N-ras were found to be clonal. One of the remaining 32 proviral integration site sequences that show no similarities to known sequences may represent a common integration site, as 2 of the 20 tumors demonstrated clonal provirus insertion into this region.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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DNA, Neoplasm / genetics*
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Genes, MHC Class II
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Genes, myc
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Genes, ras
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Leukemia Virus, Murine / genetics*
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Lymphoma, T-Cell / genetics
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Lymphoma, T-Cell / virology*
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Mice
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Molecular Sequence Data
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Polymerase Chain Reaction
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Protein Kinase C / genetics
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Protein Serine-Threonine Kinases*
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins c-pim-1
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Receptors, Antigen, T-Cell, alpha-beta / genetics
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Repetitive Sequences, Nucleic Acid
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Virus Integration*
Substances
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DNA, Neoplasm
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Proto-Oncogene Proteins
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Receptors, Antigen, T-Cell, alpha-beta
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Pim1 protein, mouse
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Protein Serine-Threonine Kinases
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Proto-Oncogene Proteins c-pim-1
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Protein Kinase C
Associated data
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GENBANK/Z69798
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GENBANK/Z69799
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GENBANK/Z69800
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GENBANK/Z69801
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GENBANK/Z69802
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GENBANK/Z69803
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GENBANK/Z69804
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GENBANK/Z69805
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GENBANK/Z69806
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GENBANK/Z69807
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GENBANK/Z69808
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GENBANK/Z69809
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GENBANK/Z69810
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GENBANK/Z69811
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GENBANK/Z69812
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GENBANK/Z69813
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GENBANK/Z69814
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GENBANK/Z69815
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GENBANK/Z69816
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GENBANK/Z69817
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GENBANK/Z69818
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GENBANK/Z69819
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GENBANK/Z69820
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GENBANK/Z69821
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GENBANK/Z69822
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GENBANK/Z69823
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GENBANK/Z69824
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GENBANK/Z69825
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GENBANK/Z69826
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GENBANK/Z69827