Comparative DNA analysis of two vanA plasmids from Enterococcus faecium strains isolated from poultry and a poultry farmer in Norway

H Sletvold; P J Johnsen; G S Simonsen; B Aasnaes; A Sundsfjord; K M Nielsen

doi:10.1128/AAC.00557-06

Comparative DNA analysis of two vanA plasmids from Enterococcus faecium strains isolated from poultry and a poultry farmer in Norway

Antimicrob Agents Chemother. 2007 Feb;51(2):736-9. doi: 10.1128/AAC.00557-06. Epub 2006 Nov 20.

Authors

H Sletvold¹, P J Johnsen, G S Simonsen, B Aasnaes, A Sundsfjord, K M Nielsen

Affiliation

¹ Department of Pharmacy, Faculty of Medicine, University of Tromsø, and Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway. hege.sletvold@farmasi.uit.no

Abstract

The DNA sequences of two plasmids carrying vanA, pVEF1 (39,626 bp) and pVEF2 (39,714 bp), were determined. Forty-three shared coding sequences were identified, and the only nucleotide difference was an 88-bp indel. A postsegregational killing system was identified. This system possibly explains the persistence of the vanA gene cluster in Norwegian poultry farms.

Publication types

Comparative Study

MeSH terms

Animals
Bacterial Proteins / genetics*
Carbon-Oxygen Ligases / genetics*
DNA, Bacterial / genetics
Enterococcus faecium / genetics*
Enterococcus faecium / isolation & purification
Molecular Sequence Data
Plasmids*
Poultry / microbiology*
Species Specificity

Substances

Bacterial Proteins
DNA, Bacterial
VanA ligase, Bacteria
Carbon-Oxygen Ligases

Associated data

GENBANK/AM296544
GENBANK/AM410096