Genomic, Recombinational and Phylogenetic Characterization of Global Feline Herpesvirus 1 Isolates

Andrew C Lewin; Aaron W Kolb; Gillian J McLellan; Ellison Bentley; Kristen A Bernard; Sandra P Newbury; Curtis R Brandt

doi:10.1016/j.virol.2018.03.018

Genomic, Recombinational and Phylogenetic Characterization of Global Feline Herpesvirus 1 Isolates

Virology. 2018 May:518:385-397. doi: 10.1016/j.virol.2018.03.018. Epub 2018 Mar 30.

Authors

Andrew C Lewin¹, Aaron W Kolb², Gillian J McLellan³, Ellison Bentley¹, Kristen A Bernard⁴, Sandra P Newbury⁵, Curtis R Brandt⁶

Affiliations

¹ Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Wisconsin, USA; McPherson Eye Research Institute, University of Wisconsin-Madison, Wisconsin, USA.
² Department of Ophthalmology and Visual Sciences, School of Medicine and Public Health, University of Wisconsin-Madison, Wisconsin, USA.
³ Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Wisconsin, USA; McPherson Eye Research Institute, University of Wisconsin-Madison, Wisconsin, USA; Department of Ophthalmology and Visual Sciences, School of Medicine and Public Health, University of Wisconsin-Madison, Wisconsin, USA.
⁴ Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Wisconsin, USA.
⁵ Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Wisconsin, USA.
⁶ McPherson Eye Research Institute, University of Wisconsin-Madison, Wisconsin, USA; Department of Ophthalmology and Visual Sciences, School of Medicine and Public Health, University of Wisconsin-Madison, Wisconsin, USA; Department of Medical Microbiology and Immunology, School of Medicine and Public Health, University of Wisconsin-Madison, Wisconsin, USA. Electronic address: crbrandt@wisc.edu.

Abstract

Feline herpes virus type 1 (FHV-1) is widely considered to be the leading cause of ocular disease in cats and has been implicated in upper respiratory tract infections. Little, however is known about interstrain phylogenetic relationships, and details of the genomic structure. For the present study, twenty-six FHV-1 isolates from different cats in animal shelters were collected from eight separate locations in the USA, and the genomes sequenced. Genomic characterization of these isolates includied short sequence repeat (SSR) detection, with fewer SSRs detected, compared to herpes simplex viruses type 1 and 2. For phylogenetic and recombination analysis, 27 previously sequenced isolates of FHV-1 were combined with the 26 strains sequenced for the present study. The overall genomic interstrain genetic distance between all available isolates was 0.093%. Phylogenetic analysis identified four main FHV-1 clades primarily corresponding to geographical collection site. Recombination analysis suggested that interclade recombination has occurred.

Keywords: feline herpesvirus; genome sequencing; herpes; ocular; phylogenetics; recombination; varicellovirus; veterinary.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cat Diseases / virology
Cats
Genetic Variation*
Genome, Viral*
Global Health
Herpesviridae Infections / veterinary
Phylogeny*
Recombination, Genetic*
Sequence Analysis, DNA
Varicellovirus / classification*
Varicellovirus / genetics*
Varicellovirus / isolation & purification

Supplementary concepts

Felid alphaherpesvirus 1

Abstract

Publication types

MeSH terms

Supplementary concepts

Grants and funding