Hybridoma fusion cell lines contain an aberrant kappa transcript

Mol Immunol. 1988 Oct;25(10):991-5. doi: 10.1016/0161-5890(88)90005-3.

Abstract

The V region sequence of a non-productive kappa transcript from two myeloma fusion partners has been determined. This transcript has an aberrant VJ recombination site resulting in a translation stop site at position 105. It is variably expressed in hybridomas made from all fusion partners derived from the original MOPC-21 tumor. The amount of this transcript may greatly exceed levels of the productive light chain mRNA.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Neoplasm / genetics
  • Base Sequence
  • Cell Line
  • Genes, Immunoglobulin
  • Humans
  • Hybridomas / immunology*
  • Immunoglobulin kappa-Chains / genetics*
  • Mice
  • Molecular Sequence Data
  • Multiple Myeloma / genetics
  • Transcription, Genetic*

Substances

  • Antibodies, Neoplasm
  • Immunoglobulin kappa-Chains