Abstract
We here describe the identification and characterization of a novel bromodomain-containing protein, the bromodomain protein of 75 kDa (BP75). Initially, we identified BP75 in a two-hybrid screening for proteins that interact with the first PDZ (acronym for post-synaptic density protein PSD-95, Drosophila discs large tumor suppressor DlgA and the tight junction protein ZO-1) domain in protein tyrosine phosphatase-BAS-like (PTP-BL). We found that BP75 is expressed ubiquitously and show that both BP75 and a PTP-BL deletion mutant consisting of the first PDZ domain are located mainly in the nucleus, although cytoplasmic localization is also evident. Full-length PTP-BL, on the contrary, is predominantly localized in the cytoplasm, although some basal nuclear staining is observed. The described molecular interaction may reflect a mechanism of coupling submembraneous signalling events and nuclear events.
MeSH terms
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3T3 Cells
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Amino Acid Sequence
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Animals
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Bromine / chemistry*
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COS Cells
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Carrier Proteins / genetics*
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Carrier Proteins / isolation & purification
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Carrier Proteins / metabolism
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Chromosomal Proteins, Non-Histone*
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Conserved Sequence*
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DNA, Complementary / metabolism
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Disks Large Homolog 4 Protein
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Guanylate Kinases
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins
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Mice
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Mice, Inbred C57BL
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Molecular Sequence Data
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Nerve Tissue Proteins / metabolism
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Nuclear Proteins / genetics*
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Nuclear Proteins / isolation & purification
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Nuclear Proteins / metabolism
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Protein Structure, Tertiary
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Protein Tyrosine Phosphatase, Non-Receptor Type 13
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Protein Tyrosine Phosphatases / chemistry
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Protein Tyrosine Phosphatases / metabolism
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Sequence Homology, Amino Acid
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Subcellular Fractions / metabolism
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Two-Hybrid System Techniques
Substances
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Brd7 protein, mouse
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Carrier Proteins
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Chromosomal Proteins, Non-Histone
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DNA, Complementary
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Disks Large Homolog 4 Protein
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Dlg4 protein, mouse
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins
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Nerve Tissue Proteins
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Nuclear Proteins
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postsynaptic density proteins
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Guanylate Kinases
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Protein Tyrosine Phosphatase, Non-Receptor Type 13
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Protein Tyrosine Phosphatases
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Ptpn13 protein, mouse
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Bromine