Identification and molecular characterization of BP75, a novel bromodomain-containing protein

FEBS Lett. 1999 Oct 15;459(3):291-8. doi: 10.1016/s0014-5793(99)01191-6.

Abstract

We here describe the identification and characterization of a novel bromodomain-containing protein, the bromodomain protein of 75 kDa (BP75). Initially, we identified BP75 in a two-hybrid screening for proteins that interact with the first PDZ (acronym for post-synaptic density protein PSD-95, Drosophila discs large tumor suppressor DlgA and the tight junction protein ZO-1) domain in protein tyrosine phosphatase-BAS-like (PTP-BL). We found that BP75 is expressed ubiquitously and show that both BP75 and a PTP-BL deletion mutant consisting of the first PDZ domain are located mainly in the nucleus, although cytoplasmic localization is also evident. Full-length PTP-BL, on the contrary, is predominantly localized in the cytoplasm, although some basal nuclear staining is observed. The described molecular interaction may reflect a mechanism of coupling submembraneous signalling events and nuclear events.

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Bromine / chemistry*
  • COS Cells
  • Carrier Proteins / genetics*
  • Carrier Proteins / isolation & purification
  • Carrier Proteins / metabolism
  • Chromosomal Proteins, Non-Histone*
  • Conserved Sequence*
  • DNA, Complementary / metabolism
  • Disks Large Homolog 4 Protein
  • Guanylate Kinases
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Nerve Tissue Proteins / metabolism
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / isolation & purification
  • Nuclear Proteins / metabolism
  • Protein Structure, Tertiary
  • Protein Tyrosine Phosphatase, Non-Receptor Type 13
  • Protein Tyrosine Phosphatases / chemistry
  • Protein Tyrosine Phosphatases / metabolism
  • Sequence Homology, Amino Acid
  • Subcellular Fractions / metabolism
  • Two-Hybrid System Techniques

Substances

  • Brd7 protein, mouse
  • Carrier Proteins
  • Chromosomal Proteins, Non-Histone
  • DNA, Complementary
  • Disks Large Homolog 4 Protein
  • Dlg4 protein, mouse
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Nuclear Proteins
  • postsynaptic density proteins
  • Guanylate Kinases
  • Protein Tyrosine Phosphatase, Non-Receptor Type 13
  • Protein Tyrosine Phosphatases
  • Ptpn13 protein, mouse
  • Bromine

Associated data

  • GENBANK/AF084259