Abstract
From the roots of Salvia prionitis a new tricyclic diterpene, saprirearine (1), a new anhydride-type compound, saprionide (2), a new 7,8-seco-abietane diterpene derivative, 7,8-seco-para-ferruginone (3), and two new 4,5-seco-5,10-friedo-abietane diterpenoids, 4-hydroxysaprorthoquinone (4) and 3-keto-4-hydroxysaprorthoquinone (5), were isolated. Their structures were established by spectroscopic methods and chemical transformation. Compound 3 showed antimicrobial activities against two Gram-positive organisms, Staphylococcus aureus and Micrococcus luteus, with MIC values of 20.0 and 15.0 microM, respectively. Compound 4 showed significant inhibition against topoisomerase Iota with an IC50 value of 0.8 microM. Compound 5 exhibited cytotoxic activities against HL-60 human leukemia and the SGC-7901 and MKN-28 stomach cancer cell lines, with IC50 values of 4.6, 0.2, and 0.3 microM, respectively.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / isolation & purification*
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Anti-Bacterial Agents / pharmacology
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / isolation & purification*
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Antineoplastic Agents, Phytogenic / pharmacology
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Chromatography, Thin Layer
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DNA Topoisomerases, Type I
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Diterpenes / chemistry
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Diterpenes / isolation & purification*
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Diterpenes / pharmacology
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Drug Screening Assays, Antitumor
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Drugs, Chinese Herbal / chemistry
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Drugs, Chinese Herbal / isolation & purification*
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Drugs, Chinese Herbal / pharmacology
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Gram-Positive Bacteria / drug effects*
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HL-60 Cells / drug effects
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Humans
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Inhibitory Concentration 50
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Microbial Sensitivity Tests
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Micrococcus luteus / drug effects
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Molecular Conformation
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Molecular Structure
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Plant Roots / chemistry
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Plants, Medicinal / chemistry*
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Salvia / chemistry*
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Staphylococcus aureus / drug effects
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Stereoisomerism
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Stomach Neoplasms
Substances
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7,8-seco-para-ferruginone
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Anti-Bacterial Agents
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Antineoplastic Agents, Phytogenic
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Diterpenes
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Drugs, Chinese Herbal
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saprionide
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saprirearine
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DNA Topoisomerases, Type I