Substrate specificity of thermostable farnesyl diphosphate synthase with alkyl group homologs of isopentenyl diphosphate

Bioorg Med Chem Lett. 1998 Sep 22;8(18):2549-54. doi: 10.1016/s0960-894x(98)00458-2.

Abstract

3-Alkyl group homologs of isopentenyl diphosphate were examined for the reactivity as substrates of the thermostable farnesyl diphosphate (FPP) synthase of Bacillus stearothermophilus. Even 3-n-propyl- and 3-n-butyl-but-3-enyl diphosphates, which are hardly acceptable by animal FPP synthases, are accepted by this bacterial enzyme as substrates to react with dimethylallyl- and geranyl diphosphates, yielding 7-methyl-3-n-propylocta-2,6-dienyl- and 7,11-dimethyl-3-n-propyldodeca-2,6,10-trienyl diphosphate, respectively.

MeSH terms

  • Alkyl and Aryl Transferases / metabolism*
  • Alkylation
  • Animals
  • Antigens, Bacterial / chemistry*
  • Antigens, Bacterial / metabolism
  • Chromatography, High Pressure Liquid
  • Geobacillus stearothermophilus
  • Geranyltranstransferase
  • Hemiterpenes*
  • Liver / enzymology
  • Organophosphorus Compounds / chemistry*
  • Organophosphorus Compounds / metabolism
  • Substrate Specificity
  • Swine

Substances

  • Antigens, Bacterial
  • Hemiterpenes
  • Organophosphorus Compounds
  • isopentenyl pyrophosphate
  • Alkyl and Aryl Transferases
  • Geranyltranstransferase