Abstract
3-Alkyl group homologs of isopentenyl diphosphate were examined for the reactivity as substrates of the thermostable farnesyl diphosphate (FPP) synthase of Bacillus stearothermophilus. Even 3-n-propyl- and 3-n-butyl-but-3-enyl diphosphates, which are hardly acceptable by animal FPP synthases, are accepted by this bacterial enzyme as substrates to react with dimethylallyl- and geranyl diphosphates, yielding 7-methyl-3-n-propylocta-2,6-dienyl- and 7,11-dimethyl-3-n-propyldodeca-2,6,10-trienyl diphosphate, respectively.
MeSH terms
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Alkyl and Aryl Transferases / metabolism*
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Alkylation
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Animals
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Antigens, Bacterial / chemistry*
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Antigens, Bacterial / metabolism
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Chromatography, High Pressure Liquid
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Geobacillus stearothermophilus
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Geranyltranstransferase
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Hemiterpenes*
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Liver / enzymology
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Organophosphorus Compounds / chemistry*
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Organophosphorus Compounds / metabolism
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Substrate Specificity
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Swine
Substances
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Antigens, Bacterial
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Hemiterpenes
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Organophosphorus Compounds
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isopentenyl pyrophosphate
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Alkyl and Aryl Transferases
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Geranyltranstransferase