Inhibitory effects of 9-beta-D-xylofuranosyladenine 5'-triphosphate on DNA-dependent RNA polymerase I and II from cherry salmon (Oncorhynchus masou)

J Biochem. 1985 May;97(5):1385-9. doi: 10.1093/oxfordjournals.jbchem.a135192.

Abstract

In order to determine the mode of action of cytostatic 9-beta-D-xylofuranosyladenine (xylo-A), the inhibitory effects of 9-beta-D-xylofuranosyladenine 5'-triphosphate (xylo-ATP) on DNA-dependent RNA polymerases I and II purified from cherry salmon (Oncorhynchus masou) liver nuclei were studied. This nucleotide showed strong inhibitory action on both RNA polymerases I and II. The K1 values are 14 microM for polymerase I and 5 microM for polymerase II (Km values of ATP are 37 microM for polymerase I and 40 microM for polymerase II). The mode of xylo-ATP was competitive with respect to the incorporation of AMP into RNA and non-competitive to UTP and CTP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / analogs & derivatives*
  • Adenosine Triphosphate / pharmacology
  • Animals
  • Deoxyadenine Nucleotides / pharmacology
  • Kinetics
  • Liver / enzymology
  • RNA / biosynthesis
  • RNA Polymerase I / antagonists & inhibitors*
  • RNA Polymerase II / antagonists & inhibitors*
  • Ribonucleotides / metabolism
  • Transcription, Genetic / drug effects

Substances

  • Deoxyadenine Nucleotides
  • Ribonucleotides
  • 3'-deoxyadenosine 5'-triphosphate
  • RNA
  • 9-beta-xylofuranosyladenine 5'-triphosphate
  • Adenosine Triphosphate
  • RNA Polymerase II
  • RNA Polymerase I