Identification of enteric pathogens by heat shock protein 60 kDa (HSP60) gene sequences

FEMS Microbiol Lett. 2002 Jan 2;206(1):107-13. doi: 10.1111/j.1574-6968.2002.tb10994.x.

Abstract

A highly specific and reproducible approach for the simultaneous detection of enteric pathogenic bacteria was developed using bacterial hsp60 gene and molecular biological tools. A single pair of universal primers was derived from the highly conserved sequence of hsp60 genes encompassing a 600-bp hypervariable region. PCR amplification followed by either dot blot hybridization or restriction enzyme digestion performed on 38 enteric bacteria indicated that this approach could differentiate not only different genera such as Campylobacter, Yersinia and Vibrio, but also species that are closely related genetically, such as between C. jejuni and C. coli, or between Salmonella and Shigella or Escherichia coli.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence*
  • Chaperonin 60 / genetics*
  • Diarrhea / diagnosis
  • Diarrhea / microbiology*
  • Gram-Negative Bacteria / classification*
  • Gram-Negative Bacteria / genetics
  • Gram-Negative Bacteria / isolation & purification
  • Gram-Negative Bacterial Infections / diagnosis
  • Gram-Negative Bacterial Infections / microbiology*
  • Humans
  • Immunoblotting
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Analysis, DNA

Substances

  • Chaperonin 60

Associated data

  • GENBANK/AF230935
  • GENBANK/AF230951
  • GENBANK/AF230955
  • GENBANK/AF230959
  • GENBANK/AF230960
  • GENBANK/AY044098
  • GENBANK/AY044099
  • GENBANK/AY044100
  • GENBANK/AY044101
  • GENBANK/AY044102
  • GENBANK/AY044103
  • GENBANK/AY044104
  • GENBANK/AY044105
  • GENBANK/AY044106