Abstract
The Kento-Mwana project was carried out in Pointe Noire, Republic of the Congo, to prevent mother-to-child HIV-1 transmission. To determine the prevalence of different subtypes and transmitted drug resistance-associated mutations, 95 plasma samples were collected at baseline from HIV-1-positive naive pregnant women enrolled in the project during the years 2005-2008. Full protease and partial reverse transcriptase sequencing was performed and 68/95 (71.6%) samples were successfully sequenced. Major mutations to nucleoside reverse transcriptase inhibitors, nonnucleoside reverse transcriptase inhibitors, and protease inhibitors were detected in 4/68 (5.9%), 3/68 (4.4%), and 2/68 (2.9%) samples, respectively. Phylogenetic analysis of HIV-1 isolates showed a high prevalence of unique recombinant forms (24/68, 35%), followed by CRF45_cpx (7/68, 10.3%) and subsubtype A3 and subtype G (6/68 each, 8.8%). Although the prevalence of transmitted drug resistance mutations appears to be currently limited, baseline HIV-1 genotyping is highly advisable in conjunction with antiretroviral therapy scale-up in resource-limited settings to optimize treatment and prevent perinatal transmission.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cluster Analysis
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Congo / epidemiology
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Drug Resistance, Viral*
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Female
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Genotype
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HIV Infections / epidemiology
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HIV Infections / virology*
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HIV Protease / genetics
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HIV Reverse Transcriptase / genetics
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HIV-1 / classification*
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HIV-1 / drug effects*
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HIV-1 / genetics
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HIV-1 / isolation & purification
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Humans
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Infant, Newborn
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Molecular Sequence Data
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Mutation*
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Phylogeny
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Pregnancy
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Pregnancy Complications, Infectious / epidemiology
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Pregnancy Complications, Infectious / virology*
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Prevalence
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Sequence Analysis, DNA
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Sequence Homology
Substances
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reverse transcriptase, Human immunodeficiency virus 1
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HIV Reverse Transcriptase
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HIV Protease
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p16 protease, Human immunodeficiency virus 1
Associated data
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GENBANK/EU914159
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GENBANK/EU914160
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GENBANK/EU914161
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GENBANK/EU914162
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GENBANK/EU914163
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GENBANK/EU914164
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GENBANK/EU914165
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GENBANK/EU914166
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GENBANK/EU914167
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GENBANK/EU914168
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GENBANK/EU914169
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GENBANK/EU914170
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GENBANK/EU914171
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GENBANK/EU914172
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GENBANK/EU914173
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GENBANK/EU914174
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GENBANK/EU914175
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GENBANK/EU914176
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GENBANK/EU914177
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GENBANK/EU914178
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GENBANK/EU914179
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GENBANK/EU914180
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GENBANK/EU914181
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GENBANK/EU914182
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GENBANK/EU914183
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GENBANK/EU914184
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GENBANK/EU914185
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GENBANK/EU914186
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GENBANK/EU914187
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GENBANK/KP889062
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