An infectious molecular clone of an unusual macrophage-tropic and highly cytopathic strain of human immunodeficiency virus type 1

J Virol. 1992 Dec;66(12):7517-21. doi: 10.1128/JVI.66.12.7517-7521.1992.

Abstract

We isolated and molecularly cloned a human immunodeficiency virus type 1 (HIV-1) strain (89.6) which is unusual because it is both macrophage-tropic and extremely cytopathic in lymphocytes. Moreover, this is the first well-characterized infectious molecularly cloned macrophage-tropic HIV-1 strain derived from peripheral blood. HIV-1 89.6 differs markedly from other macrophage-tropic isolates within the envelope V3 region, which is important in determining cell tropism and cytopathicity. HIV-1 89.6 may thus represent a transitional isolate between noncytopathic macrophage-tropic viruses and cytopathic lymphocyte-tropic viruses.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Cell Line
  • Cloning, Molecular / methods*
  • DNA, Viral / genetics
  • DNA, Viral / isolation & purification
  • Genes, Viral
  • HIV Long Terminal Repeat
  • HIV-1 / genetics*
  • HIV-1 / isolation & purification
  • HIV-1 / physiology*
  • Humans
  • Macrophages / microbiology*
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Open Reading Frames
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • T-Lymphocytes
  • Viral Proteins / genetics
  • Virus Replication

Substances

  • DNA, Viral
  • Viral Proteins

Associated data

  • GENBANK/M96155