Antibody repertoire and gene expression profile: implications for different developmental and functional traits of splenic and peritoneal B-1 lymphocytes

J Immunol. 2003 Aug 1;171(3):1192-201. doi: 10.4049/jimmunol.171.3.1192.

Abstract

In L2 mice, a high expression level of the transgenic lambda2(315) L chain results in nearly complete exclusion of endogenous L chains and a predominance of B-1a cells. In this study, we show that splenic and peritoneal B-1a cells differ considerably in their Ab repertoire and gene expression profile. Splenic B-1a cells exhibit a more diversified repertoire under L chain limitation. Despite oligoclonal overlaps between both B-1a compartments, some B cell receptor specificities are clearly restricted to the peritoneum. The capacity of peritoneal B-1a cells to enter the splenic B-1a compartment was found to be very limited. Gene expression profiling revealed genes up-regulated in splenic B-1a cells that are involved in mediating specialized first-line-of-defense effector functions and interaction with T cells. Thus, splenic and peritoneal B-1a cells differ not only in their developmental program but also in functional properties.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibody Diversity / genetics
  • Antibody Specificity / genetics
  • Antigens, CD / biosynthesis
  • Ascitic Fluid / cytology
  • Ascitic Fluid / immunology*
  • Ascitic Fluid / metabolism
  • B-Lymphocyte Subsets / cytology
  • B-Lymphocyte Subsets / immunology*
  • B-Lymphocyte Subsets / metabolism
  • B7-1 Antigen / biosynthesis
  • B7-2 Antigen
  • Base Sequence
  • Cell Differentiation / genetics
  • Cell Differentiation / immunology
  • Cell Movement / genetics
  • Cell Movement / immunology
  • Clone Cells
  • Epitopes, B-Lymphocyte / immunology
  • Gene Expression Profiling* / methods
  • Immunoglobulin Heavy Chains / biosynthesis*
  • Immunoglobulin Heavy Chains / blood
  • Immunoglobulin Heavy Chains / genetics*
  • Immunoglobulin Variable Region / biosynthesis*
  • Immunoglobulin Variable Region / blood
  • Immunoglobulin Variable Region / genetics*
  • Membrane Glycoproteins / biosynthesis
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout
  • Mice, Transgenic
  • Molecular Sequence Data
  • Multigene Family / immunology
  • Phosphatidylcholines / immunology
  • Species Specificity
  • Spleen / cytology
  • Spleen / immunology*
  • Spleen / metabolism
  • Up-Regulation / genetics
  • Up-Regulation / immunology

Substances

  • Antigens, CD
  • B7-1 Antigen
  • B7-2 Antigen
  • Cd86 protein, mouse
  • Epitopes, B-Lymphocyte
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Variable Region
  • Membrane Glycoproteins
  • Phosphatidylcholines

Associated data

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