Identification and expression of a new sulfhydryl oxidase SOx-3 during the cell cycle and the estrus cycle in uterine cells

Biochem Biophys Res Commun. 2001 Sep 14;287(1):83-91. doi: 10.1006/bbrc.2001.5440.

Abstract

Using differential hybridization of a guinea pig endometrial cell cDNA library, a potentially negatively estrogen-regulated gene, SOX-3, was isolated. According to the nucleotide and protein sequence similarities, SOx-3 belonged to the FAD-linked sulfhydryl oxidase family containing the egg white sulfhydryl oxidase, the rat seminal vesicle sulfhydryl oxidase-2 SOx-2, the quiescence-inducible protein hQ6. The SOX-3 transcript in the guinea pig as well as 5 different mRNAs in human tissues appeared differentially expressed in the tissues studied. In secondary endometrial cell culture, the SOX-3 mRNA level increased during a serum depletion-induced quiescence, decreased when cells enter the G1 phase after serum stimulation, and was restored during the S and G2/M phases. Thus, SOX-3 could be implicated in the negative cell cycle control. The SOx-3 protein appeared to be specific of epithelial cells in the uterus. Its expression level varied during the estrus cycle in the guinea pig, suggesting a regulation by steroid hormones.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Cycle / physiology
  • DNA, Complementary / analysis
  • DNA-Binding Proteins / chemistry
  • Endometrium / metabolism
  • Estrogens / pharmacology
  • Estrus / metabolism*
  • Female
  • Gene Expression Regulation, Enzymologic
  • Gene Library
  • Guinea Pigs
  • HMGB Proteins
  • Humans
  • Molecular Sequence Data
  • Nuclear Proteins / chemistry
  • Organ Specificity
  • Oxidoreductases / biosynthesis
  • Oxidoreductases / genetics
  • Oxidoreductases / isolation & purification*
  • RNA, Messenger / drug effects
  • RNA, Messenger / metabolism
  • Rats
  • SOXB1 Transcription Factors
  • Sequence Homology, Amino Acid
  • Transcription Factors
  • Uterus / cytology
  • Uterus / enzymology*

Substances

  • DNA, Complementary
  • DNA-Binding Proteins
  • Estrogens
  • HMGB Proteins
  • Nuclear Proteins
  • RNA, Messenger
  • SOX2 protein, human
  • SOXB1 Transcription Factors
  • Sox2 protein, rat
  • Transcription Factors
  • Oxidoreductases
  • sulfhydryl oxidase

Associated data

  • GENBANK/U82982