Abstract
Yersinia pseudotuberculosis is a foodborne pathogen often detected and identified using polymerase chain reaction (PCR) with primers targeted to virulence genes. Sequence variability of the virulence genes in strains representing different serotypes is unknown. Sequence variability could hinder the recognition of this pathogen by PCR and affect the host-pathogen interactions. Sequencing of inv, virF, and yadA of 18 Y. pseudotuberculosis strains showed limited variability of inv and virF, whereas the sequences of yadA varied considerably.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adhesins, Bacterial / genetics
-
Amino Acid Sequence
-
Animals
-
Bacterial Proteins / genetics
-
Base Sequence
-
DNA Primers / genetics
-
DNA, Bacterial / chemistry
-
DNA, Bacterial / genetics
-
Foodborne Diseases / microbiology*
-
Genetic Variation / genetics*
-
Host-Pathogen Interactions
-
Humans
-
Molecular Sequence Data
-
Phylogeny
-
Polymerase Chain Reaction
-
Sensitivity and Specificity
-
Sequence Analysis, DNA
-
Species Specificity
-
Virulence Factors / chemistry
-
Virulence Factors / genetics*
-
Yersinia pseudotuberculosis / genetics*
-
Yersinia pseudotuberculosis / pathogenicity
-
Yersinia pseudotuberculosis Infections / microbiology*
Substances
-
Adhesins, Bacterial
-
Bacterial Proteins
-
DNA Primers
-
DNA, Bacterial
-
Virulence Factors
-
YadA protein, Yersinia